Περίληψη:
Grapevine is one of the most important fruit species in the world. In order to better understand genetic
basis of traits variation and facilitate the breeding of new genotypes, we sequenced, assembled, and
annotated the genome of the American native Vitis riparia, one of the main species used worldwide
for rootstock and scion breeding. A total of 164Gb raw DNA reads were obtained from Vitis riparia
resulting in a 225X depth of coverage. We generated a genome assembly of the V. riparia grape de
novo using the PacBio long-reads that was phased with the 10x Genomics Chromium linked-reads. At
the chromosome level, a 500Mb genome was generated with a scafold N50 size of 1Mb. More than
34% of the whole genome were identifed as repeat sequences, and 37,207 protein-coding genes were
predicted. This genome assembly sets the stage for comparative genomic analysis of the diversifcation
and adaptation of grapevine and will provide a solid resource for further genetic analysis and breeding
of this economically important species.
Αναφορά NLM:
Girollet N, Rubio B, Lopez-Roques C, Valière S, Ollat N, Bert PF. De novo phased assembly of the Vitis riparia grape genome. Sci Data. 2019 Jul 19;6(1):127. doi: 10.1038/s41597-019-0133-3. Erratum in: Sci Data. 2019 Oct 30;6(1):249. PMID: 31324816; PMCID: PMC6642119.